The image is the furthest photo away from Earth ever taken from outer-space.
New Horizons flew past the dwarf planet Pluto in July 2015.
After the fly-by, the spacecraft continued into the Kuiper Belt.
The released photo surpassed the “Pale Blue Dot” images of Earth that were taken in 1990 by NASA’s Voyager 1.
The images for “Pale Blue Dot” – part of a composite – were taken from 3.75 billion miles (6.06 billion kilometres) away.
New Horizons took more photos as it sped deeper into the cosmos in December.
These pictures show two objects in the Kuiper Belt, the so-called twilight zone on the fringes of our solar system.
NASA released the images this week.
The US Space Agency said: ”That New Year’s flight past MU69 will be the farthest planetary encounter in history, happening one billion miles beyond the Pluto system – which New Horizons famously explored in July 2015.”
Lead scientist Alan Stern of the Southwest Research Institute in Boulder, Colorado, said: “New Horizons just could not be better… we are bearing down on our flyby target.”
New Horizons is only the fifth man-made spacecraft to ever travel beyond the outer planets.
Many of the spacecraft’s activities are setting distance records, according to NASA.
New Horizons was launched in 2006 and is currently in electronic hibernation.
The spacecraft is set to head 1 billion miles (1.6 billion kilometres) beyond Pluto, on January 1 2019.
Flight controllers at a Johns Hopkins University lab in Laurel, Maryland, will awaken the spacecraft in June and begin preparing it for the flyby.